Wednesday, August 10, 2011

I Need Chloride as much as Everyone Else Needs Agar

We were almost out of Artificial Sea Water...again. So I decided to make 10 liters of ASW: enough to last at least a couple of weeks. The problem was that it called for 10 times the amount of chlorides and other compounds that go into making ASW. It seems like we have to work awful hard in order to imitate nature. As a refresher, here is the line-up of what goes into making artificial ocean (for full recipe, click here):

From left to right: Sodium Chloride, Tris Base, Calcium Chloride, Potassium Chloride, Magnesium Chloride, Sodium Sulfate.

Once those compounds are solubilized in MilliQ (super-purified, de-ionized) water, then the pH must be adjusted until it is within the 8.1 to 8.2 range. Of course, the problem today was that we ran out of Magnesium Chloride. Humorously enough, I did find three empty containers of it, but sometimes things just get used up really quickly when they're vital to whatever experiments might be going on.

A Weighing Tray with approximately 40 grams of Magnesium Chloride on it.




Thus, after I finished measuring out all the other 'ingredients' and adding them to the MilliQ water, I was left with a Magnesium Chloride-deficient solution; destined to mix and solubilize atop the magnetic stirring plate (above).



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